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Evidence-based GLP-1 & peptide discussion since 2023
ForumsCOA & Analytical TestingBatch-to-batch purity variation — acceptable ranges Page 3

Batch-to-batch purity variation — acceptable ranges

kate.chem Sat, Jun 6, 2026 at 4:15 AM 15 replies 505 viewsPage 3 of 3
nick_newbie
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Jun 6, 2026 at 7:33 PM#11
Dr.PulmRoch said:
They are not contradictory, they measure different things, and this is the single most common misreading of a certificate.

Saving this. It is the first explanation that did not require me to already understand it. Printing the relevant bit and taking it with me.

22 20robert_kc, dan_philly, MeganSA_TX and 19 others
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JenPlateau
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Jun 7, 2026 at 6:12 AM#12

Clinical perspective, offered as context rather than as advice.

Community-verified vendors for certificates of analysis: I maintain a spreadsheet of Janoshik-verified test results submitted by community members. Across 48 submitted tests:

  • 85% tested at ≥98% purity
  • 10% tested at 95-98% purity
  • 5% tested at <95% purity (concerning)

The vendors with consistently high purity results are listed in the Vendor Directory. We update monthly based on new community submissions.

23 21TrialNerd_Beth, HPLC_Greg, LibrarianMeg and 20 others
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TirzTom
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Jun 7, 2026 at 4:51 PM#13
PharmHunterJen said:
HPLC results interpretation for certificates of analysis: when you get your Janoshik report back, here's what the numbers mean: Purity %: Should be…

Third-party testing services for certificates of analysis besides Janoshik:

  • Janoshik: Gold standard for peptide community, ~$121, 10-14 day turnaround
  • Valitech: US-based, ISO 17025 accredited, ~$201
  • Eurofins: Full pharmaceutical testing suite, more expensive but comprehensive
  • ChemTox: Basic identity and purity testing, ~$121

Janoshik is the community standard but any ISO-accredited analytical lab can verify peptide identity and purity. The key is independence — don't rely solely on the vendor's own testing.

24 22Dr.EndoIndy, tom_AK, josh_phd_bmore and 21 others
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Sigma-Aldrich — Research-Grade Standards

Certified reference materials, analytical reagents, and research-grade standards for peptide verification. Trusted by laboratories worldwide.

Shop Reference Standards
bri_stats
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Jun 8, 2026 at 3:29 AM#14
kate.chem said:
The provenance questions are more useful than the numbers.

My HPLC chromatogram analysis for certificates of analysis — I sent my batch to Janoshik and here's what the results showed:

Main peak retention time: 15.4 minutes (consistent with semaglutide reference standard)
Peak purity: 98.7%
Related substances: 0.6% (single impurity at 9.2 minutes — likely a synthesis intermediate)
Mass spec: m/z 4113.6 (matches expected molecular weight)

This is a clean result. The mass spec confirmation is particularly important — it proves the molecule is actually semaglutide and not a substitution.

25 23JessicaM_2024, TomFromTexas, mike.trainer_LA and 22 others
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Admin
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Jun 8, 2026 at 2:07 PM#15

Moderator note: two posts asking for a source have been merged into one. Please search the thread before asking again. Thread quality here is what the rules are for. Keep it up.

Last edited: Jun 8, 2026 at 6:07 PM
26 24PeptideSynthNJ, Dr.KarenChen, Dr.NateNeph and 23 others
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