🍪 CompoundTalk uses cookies to improve your experience, analyze traffic, and personalize content. By continuing to use this site, you agree to our Cookie Policy.
Evidence-based GLP-1 & peptide discussion since 2023
ForumsCOA & Analytical TestingSterility testing per USP <71> — need advice

Sterility testing per USP <71> — need advice

Dr.RaviCardio Thu, Apr 9, 2026 at 9:46 AM 13 replies 672 viewsPage 1 of 3
Dr.RaviCardio
VIP Member
2,890
15,678
Jan 2024
New York, NY
Apr 9, 2026 at 9:46 AM#1

Two reports on the same product from two laboratories, four percentage points apart, and no method stated on either.

The narrow version of the question is whether a purity figure and a content figure can disagree without one of them being wrong.

Tell me what I have not thought of.

15 10pete_manc_UK, anna.melb_AU, mark_tokyo and 12 others
Reply Quote Save Share Report
NurseKim_ATL
Senior Member
1,678
7,234
Feb 2024
Atlanta, GA
Apr 9, 2026 at 10:38 AM#2

Taking the question as asked, rather than the general version of it. They are not contradictory, they measure different things, and this is the single most common misreading of a certificate. Purity is a relative area measurement: the main peak as a percentage of total peak area in the chromatogram. Content is an absolute mass measurement against a reference standard. A vial can be 99% pure and contain 6.1mg — pure material, overfilled — or 99% pure and contain 3mg, which is pure material and a short fill. Purity tells you what fraction of what is there is the right molecule; content tells you how much of it there is. You need both, and a certificate with only purity is telling you half the story.

Last edited: Apr 9, 2026 at 3:38 PM
14 9raj_cambridge, ingrid_STO, pete_nash and 11 others
Reply Quote Save Share Report
RetaRick_CA
VIP Member
2,012
9,876
Jan 2024
California
Apr 9, 2026 at 11:30 AM#3
NurseKim_ATL said:
They are not contradictory, they measure different things, and this is the single most common misreading of a certificate.

No disagreement with NurseKim_ATL. One condition attached. One addition: an unstated method makes a report close to uninterpretable. "99% pure" without the technique, the standard and the conditions is a claim, not a measurement.

Last edited: Apr 9, 2026 at 12:30 PM
13 8DadBodDave, AmyNC_wife, SkepticalSean and 10 others
Reply Quote Save Share Report

Sigma-Aldrich — Research-Grade Standards

Certified reference materials, analytical reagents, and research-grade standards for peptide verification. Trusted by laboratories worldwide.

Shop Reference Standards
ben_calgary
Member
245
1,123
Oct 2024
Calgary, CA
Apr 9, 2026 at 12:22 PM#4
Dr.RaviCardio said:
Two reports on the same product from two laboratories, four percentage points apart, and no method stated on either.

Can confirm the pattern Dr.RaviCardio describes. The provenance questions are more useful than the numbers. Does the report name the batch on your vial. Is the date consistent with when that batch existed. Is it addressed to the vendor or to the person who paid for the test. Was the sample taken from general stock or supplied by the vendor. A perfect certificate for a different batch is worth nothing, and vendor-supplied samples are the most common way a genuine report ends up misleading.

12 7JakeSmashed95, NauseaFreeNow, SteveThurs and 9 others
Reply Quote Save Share Report
Dr.KarenChen
VIP Member
4,210
24,567
Nov 2023
San Francisco, CA
Apr 9, 2026 at 5:19 PM#5

Clinical perspective, offered as context rather than as advice.

Third-party testing services for certificates of analysis besides Janoshik:

  • Janoshik: Gold standard for peptide community, ~$139, 10-14 day turnaround
  • Valitech: US-based, ISO 17025 accredited, ~$219
  • Eurofins: Full pharmaceutical testing suite, more expensive but comprehensive
  • ChemTox: Basic identity and purity testing, ~$109

Janoshik is the community standard but any ISO-accredited analytical lab can verify peptide identity and purity. The key is independence — don't rely solely on the vendor's own testing.

11 6sarah_nash92, FitDadDave, RunnerRach and 8 others
Reply Quote Save Share Report

Similar Threads

HPLC vs UPLC for peptide purity — method comparison study18 replies
Mass spectrometry for peptide identity verification — ESI-MS guide7 replies
Endotoxin testing methods — LAL vs recombinant Factor C17 replies
Red flags on COAs — how to spot a fake certificate5 replies
USP reference standards for peptide verification — sourcing guide3 replies
ForumsNewTrendingMembersAccount

Log In

Forgot password?
No account? Register