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ForumsCOA & Analytical TestingLC-MS/MS vs HPLC for peptide analysis — November 2025 Page 4

LC-MS/MS vs HPLC for peptide analysis — November 2025

pat_auckland Wed, Apr 1, 2026 at 10:13 AM 19 replies 1,044 viewsPage 4 of 4
Dr.LipidDallas
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Apr 7, 2026 at 6:22 AM#16

From the other side of the consultation, briefly.

Third-party testing services for certificates of analysis besides Janoshik:

  • Janoshik: Gold standard for peptide community, ~$127, 10-14 day turnaround
  • Valitech: US-based, ISO 17025 accredited, ~$247
  • Eurofins: Full pharmaceutical testing suite, more expensive but comprehensive
  • ChemTox: Basic identity and purity testing, ~$107

Janoshik is the community standard but any ISO-accredited analytical lab can verify peptide identity and purity. The key is independence — don't rely solely on the vendor's own testing.

44 17pam_stl, wei_SG, cory_ATX and 41 others
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Dr.EndoIndy
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Apr 7, 2026 at 7:17 PM#17
Dr.PulmRoch said:
HPLC results interpretation for certificates of analysis: when you get your Janoshik report back, here's what the numbers mean: Purity %: Should be…

Vendor quality matters when discussing certificates of analysis. I've tested my last 5 batches through Janoshik and purity has ranged from 98.4% to 99.2%. Consistency is key.

For anyone new: always request the COA, and consider independent verification. The $300 for a Janoshik test is worth the peace of mind.

45 18LeilaHI, marcus_mpls, DeniseRN_TPA and 42 others
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TirzTom
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Apr 8, 2026 at 8:12 AM#18
Dr.PulmRoch said:
HPLC results interpretation for certificates of analysis: when you get your Janoshik report back, here's what the numbers mean: Purity %: Should be…

COA red flags for certificates of analysis — here's what to look for when reviewing a Certificate of Analysis:

  • No HPLC data: If there's no chromatography graph, it's not a real COA
  • Exact 100% purity: Suspicious — real testing always shows slight variation
  • No batch number: Can't trace back to specific production
  • No endotoxin testing: For injectables, this is essential
  • Template looks like it was made in Word: Professional labs have standardized formats

I have seen fake COAs that are literally photoshopped. Always verify independently if possible.

46 19Dr.EndoIndy, tom_AK, josh_phd_bmore and 43 others
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Sigma-Aldrich — Research-Grade Standards

Certified reference materials, analytical reagents, and research-grade standards for peptide verification. Trusted by laboratories worldwide.

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DebRD_ATL
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Apr 8, 2026 at 9:08 PM#19

A narrower follow-up, since the general answer is now clear:

How long did you give it before you decided it was working?

Last edited: Apr 9, 2026 at 1:08 AM
47 20PedsEndoPhilly, SleepDoc_PDX, RegAffairsDC and 44 others
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mike_mod
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Apr 9, 2026 at 10:04 AM#20

Moderator note: good thread. Keeping it here rather than moving it, because the question is general enough to be useful. Carry on.

19 17PurityPaulOR, MaxMetOK, MounjBrad and 16 others
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