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Evidence-based GLP-1 & peptide discussion since 2023
ForumsCOA & Analytical TestingBatch-to-batch purity variation — looking for input Page 3

Batch-to-batch purity variation — looking for input

anders_CPH Fri, Aug 30, 2024 at 7:28 AM 19 replies 2,061 viewsPage 3 of 4
DebRD_ATL
Senior Member
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Feb 2024
Atlanta, GA
Aug 31, 2024 at 9:16 AM#11
Dr.GastroMayo said:
Reconstituted peptide stability data for certificates of analysis: I had Janoshik test my reconstituted vial at day 1, day 14, and day 28 stored in…

My vendor review relevant to certificates of analysis: ordered 5 times from my current compounder. Consistency has been excellent.

Shipping: Same-day local pickup
Packaging: Professional, discreet, proper labeling with lot/batch numbers
Purity: 99.2% average across orders (Janoshik verified)
Customer service: Responsive within 6 hours
Price: $138/month for semaglutide 1.7mg equivalent

28 1BiostatsBrad, PeptideSynthNJ, Dr.KarenChen and 25 others
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MariaRD
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Jun 2024
New Mexico
Aug 31, 2024 at 3:03 PM#12
JessicaM_2024 said:
Agreed, and mass spec answers a question purity does not: identity.

Thank you — that is the clearest version of this I have read, and I have read a lot of them. Sending this to two other people who asked me the same thing last week.

Last edited: Aug 31, 2024 at 4:03 PM
29 2anders_CPH, Dr.NutriCornell, pam_stl and 26 others
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Dr.EndoEP
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Oct 2024
El Paso, TX
Aug 31, 2024 at 8:50 PM#13
Dr.GastroMayo said:
Reconstituted peptide stability data for certificates of analysis: I had Janoshik test my reconstituted vial at day 1, day 14, and day 28 stored in…

Third-party testing services for certificates of analysis besides Janoshik:

  • Janoshik: Gold standard for peptide community, ~$126, 10-14 day turnaround
  • Valitech: US-based, ISO 17025 accredited, ~$236
  • Eurofins: Full pharmaceutical testing suite, more expensive but comprehensive
  • ChemTox: Basic identity and purity testing, ~$106

Janoshik is the community standard but any ISO-accredited analytical lab can verify peptide identity and purity. The key is independence — don't rely solely on the vendor's own testing.

30 3FranDenver, Dr.BariatricHTX, LindaRN_retired and 27 others
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Sigma-Aldrich — Research-Grade Standards

Certified reference materials, analytical reagents, and research-grade standards for peptide verification. Trusted by laboratories worldwide.

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Dr.NateNeph
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Dec 2023
Houston, TX
Sep 1, 2024 at 2:37 AM#14
anders_CPH said:
I have a certificate reporting 99.2% purity and 6.1mg content on a vial sold as 5mg, and I cannot work out whether those two numbers are consistent or…

Vendor quality matters when discussing certificates of analysis. I've tested my last 4 batches through Janoshik and purity has ranged from 98.9% to 100.5%. Consistency is key.

For anyone new: always request the COA, and consider independent verification. The $300 for a Janoshik test is worth the peace of mind.

31 4B12Beth, RickReta_CO, PharmHunterJen and 28 others
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Admin
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Sep 1, 2024 at 8:24 AM#15

Moderator note: a couple of off-topic posts removed. Report rather than reply if it drifts again.

32 5PeptideSynthNJ, Dr.KarenChen, Dr.NateNeph and 29 others
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