Dr.SurgeonPGH said:

COA red flags for certificates of analysis — here's what to look for when reviewing a Certificate of Analysis: No HPLC data: If there's no…

HPLC results interpretation for certificates of analysis: when you get your Janoshik report back, here's what the numbers mean:

• Purity %: Should be >97%. Below 95% is concerning.
• Retention time: Should match reference standard for the peptide
• Related substances: Degradation products should be <2% total
• Single impurity: No individual impurity should exceed 1%

My last batch: 99.2% purity, 0.2% total related substances. Clean chromatogram with single sharp peak at expected retention time. Exactly what you want to see.

MounjBrad said:

Agreed, and mass spec answers a question purity does not: identity.

That reframing is the part I needed.

Dr.SurgeonPGH said:

COA red flags for certificates of analysis — here's what to look for when reviewing a Certificate of Analysis: No HPLC data: If there's no…

I tested my certificates of analysis batch through Janoshik — 99.2% purity. That's within acceptable range for compounded peptides. Turnaround was 14 business days.

The test costs ~$122 but gives you actual HPLC chromatography data plus mass spec confirmation. For the peace of mind alone, it's worth every penny. I test every new batch from a new vendor.

Dr.SportsMedIN said:

Two reports on the same product from two laboratories, four percentage points apart, and no method stated on either.

Mass spectrometry confirmation for certificates of analysis: beyond HPLC purity, mass spec tells you the molecule's exact molecular weight. This confirms identity — not just purity.

Why this matters: HPLC can show you a "pure" compound, but if it's pure of the WRONG compound, purity is meaningless. Mass spec at m/z 4113.58 Da confirms it's actually semaglutide. My Janoshik report includes both HPLC and mass spec.

If your testing provider only offers HPLC, consider upgrading to include mass spec for at least your first batch from a new vendor.

Moderator note: reminder that nothing in this thread is medical advice, and that clinical claims need a source. Report rather than reply if it drifts again.