🍪 CompoundTalk uses cookies to improve your experience, analyze traffic, and personalize content. By continuing to use this site, you agree to our Cookie Policy.
Evidence-based GLP-1 & peptide discussion since 2023
ForumsCOA & Analytical TestingInter-lab variability in peptide testing — July 2024 Page 5

Inter-lab variability in peptide testing — July 2024

JennaRN Fri, Apr 5, 2024 at 2:00 AM 32 replies 2,715 viewsPage 5 of 7
NurseLeah_Nash
Member
278
1,234
Sep 2024
Nashville, TN
Apr 12, 2024 at 2:56 PM#21
Dr.AddMedPHL said:
My HPLC chromatogram analysis for certificates of analysis — I sent my batch to Janoshik and here's what the results showed: Main peak retention time:…

Community-verified vendors for certificates of analysis: I maintain a spreadsheet of Janoshik-verified test results submitted by community members. Across 51 submitted tests:

  • 75% tested at ≥98% purity
  • 18% tested at 95-98% purity
  • 7% tested at <95% purity (concerning)

The vendors with consistently high purity results are listed in the Vendor Directory. We update monthly based on new community submissions.

8 6RegAffairsDC, BiostatsBrad, PeptideSynthNJ and 5 others
Reply Quote Save Share Report
MASHdoc_SA
Member
456
2,345
Aug 2024
San Antonio, TX
Apr 14, 2024 at 9:37 PM#22
Dr.AddMedPHL said:
My HPLC chromatogram analysis for certificates of analysis — I sent my batch to Janoshik and here's what the results showed: Main peak retention time:…

Thank you for spelling out the reasoning rather than just the conclusion. Sending this to two other people who asked me the same thing last week.

9 7TrialTracker_MD, JennaRN, LabKate and 6 others
Reply Quote Save Share Report
Dr.CardioMD
VIP Member
2,678
14,567
Dec 2023
Cleveland, OH
Apr 17, 2024 at 4:17 AM#23

Clinical perspective, offered as context rather than as advice. Take it one variable at a time. Almost every unanswerable question in these threads is unanswerable because three things changed in the same fortnight, and no amount of subsequent argument can untangle them after the fact.

If somebody has the primary source to hand I would rather cite it than paraphrase it.

Last edited: Apr 17, 2024 at 10:17 AM
10 8tony_orlando, Dr.NephBHM_UK, kim_atl_prep and 7 others
Reply Quote Save Share Report

Sigma-Aldrich — Research-Grade Standards

Certified reference materials, analytical reagents, and research-grade standards for peptide verification. Trusted by laboratories worldwide.

Shop Reference Standards
tampaLisa73
Member
312
1,456
Oct 2024
Tampa, FL
Apr 19, 2024 at 10:56 AM#24
Dr.CardioMD said:
Almost every unanswerable question in these threads is unanswerable because three things changed in the same fortnight, and no amount of subsequent…

Same experience, arrived at from the opposite direction.

Last edited: Apr 19, 2024 at 11:56 AM
11 9mike.trainer_LA, sarah_nash92, FitDadDave and 8 others
Reply Quote Save Share Report
Dr.Martinez
Medical Advisor
3,891
28,456
Nov 2023
Boston, MA
Online
Apr 21, 2024 at 5:33 PM#25
Dr.CardioMD said:
Almost every unanswerable question in these threads is unanswerable because three things changed in the same fortnight, and no amount of subsequent…

Adding the part of the answer the thread has not reached. Whatever the answer turns out to be, the method for getting there is the same: state the assumption, do the arithmetic in public, and invite the correction. That is slower than asserting, and it is the only version that survives being wrong.

12 10adam_van, Dr.SurgeonPGH, rachel_ABQ and 9 others
Reply Quote Save Share Report

Similar Threads

HPLC vs UPLC for peptide purity — method comparison study18 replies
Mass spectrometry for peptide identity verification — ESI-MS guide7 replies
Endotoxin testing methods — LAL vs recombinant Factor C17 replies
Red flags on COAs — how to spot a fake certificate5 replies
USP reference standards for peptide verification — sourcing guide3 replies
ForumsNewTrendingMembersAccount

Log In

Forgot password?
No account? Register