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ForumsCOA & Analytical TestingUnderstanding peptide purity percentages — what worked for you? Page 3

Understanding peptide purity percentages — what worked for you?

LondonLisa Wed, Apr 16, 2025 at 10:14 AM 38 replies 2,088 viewsPage 3 of 8
Dr.LeslieOBGYN
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Apr 20, 2025 at 7:47 AM#11
TinaHashiRN said:
The provenance questions are more useful than the numbers.

This answered a question I did not know how to ask. Printing the relevant bit and taking it with me.

32 5Dr.BariatricHTX, LindaRN_retired, tommy_boulder and 29 others
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TinaHashiRN
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Apr 20, 2025 at 11:44 PM#12

From the other side of the consultation, briefly.

My vendor review relevant to certificates of analysis: ordered 4 times from my current compounder. Consistency has been excellent.

Shipping: 2-day priority with gel ice
Packaging: Professional, discreet, proper labeling with lot/batch numbers
Purity: 98.5% average across orders (Janoshik verified)
Customer service: Responsive within 4 hours
Price: $141/month for tirzepatide 10mg equivalent

Last edited: Apr 21, 2025 at 5:44 AM
33 6mike_mod, SarahChen_PharmD, sarah.morrison and 30 others
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DataDave
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Apr 21, 2025 at 3:41 PM#13
sophie_paris said:
Community-verified vendors for certificates of analysis: I maintain a spreadsheet of Janoshik-verified test results submitted by community members.

My HPLC chromatogram analysis for certificates of analysis — I sent my batch to Janoshik and here's what the results showed:

Main peak retention time: 14.6 minutes (consistent with semaglutide reference standard)
Peak purity: 99.6%
Related substances: 0.4% (single impurity at 8.3 minutes — likely a synthesis intermediate)
Mass spec: m/z 4113.58 (matches expected molecular weight)

This is a clean result. The mass spec confirmation is particularly important — it proves the molecule is actually semaglutide and not a substitution.

Last edited: Apr 21, 2025 at 4:41 PM
34 7LondonLisa, mike_nyc, VendorMark and 31 others
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Sigma-Aldrich — Research-Grade Standards

Certified reference materials, analytical reagents, and research-grade standards for peptide verification. Trusted by laboratories worldwide.

Shop Reference Standards
Dr.PulmRoch
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Rochester, MN
Apr 22, 2025 at 7:37 AM#14
LondonLisa said:
Inter-laboratory variation comes from real methodological differences, not sloppiness: which reference standard was used and how it was itself…

HPLC results interpretation for certificates of analysis: when you get your Janoshik report back, here's what the numbers mean:

  • Purity %: Should be >97%. Below 95% is concerning.
  • Retention time: Should match reference standard for the peptide
  • Related substances: Degradation products should be <2% total
  • Single impurity: No individual impurity should exceed 1%

My last batch: 99.5% purity, 0.1% total related substances. Clean chromatogram with single sharp peak at expected retention time. Exactly what you want to see.

Last edited: Apr 22, 2025 at 8:37 AM
35 8DebRD_ATL, KristenIndy, MarkLI_maint and 32 others
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mike_mod
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Apr 22, 2025 at 11:33 PM#15

Moderator note: a couple of off-topic posts removed. Carry on.

Last edited: Apr 23, 2025 at 1:33 AM
36 9PurityPaulOR, MaxMetOK, MounjBrad and 33 others
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