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ForumsCOA & Analytical TestingCOA red flag compilation — what worked for you? Page 2

COA red flag compilation — what worked for you?

PharmD_Rodriguez Mon, Jan 20, 2025 at 6:03 PM 28 replies 1,920 viewsPage 2 of 6
stefan_berlin
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Jan 21, 2025 at 4:45 PM#6

The figures, for anyone assembling their own picture. The four things to check on any report, in order: does it name your batch, who commissioned it, what method was used, and does it report content as well as purity. A report failing any of those is weak evidence regardless of the percentage on it.

Worth separating that from certificates of analysis, which this thread keeps folding into the same question. They behave differently and the advice does not transfer.

Last edited: Jan 21, 2025 at 7:45 PM
18 13NeuroNate, JessicaH_TX, KevinCompounds and 15 others
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PedsEndoPhilly
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Jan 22, 2025 at 1:47 AM#7

One thing that is still open after Dr.KarenChen’s answer:

What a vetting checklist should actually contain, as opposed to a list of things that are easy to fake?

17 12wendy_avl, jason_paloalto, Dr.LeslieOBGYN and 14 others
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NurseAsh_DET
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Jan 22, 2025 at 10:49 AM#8
stefan_berlin said:
The four things to check on any report, in order: does it name your batch, who commissioned it, what method was used, and does it report content as…

Adding the part of the answer the thread has not reached. They are not contradictory, they measure different things, and this is the single most common misreading of a certificate. Purity is a relative area measurement: the main peak as a percentage of total peak area in the chromatogram. Content is an absolute mass measurement against a reference standard. A vial can be 99% pure and contain 6.1mg — pure material, overfilled — or 99% pure and contain 3mg, which is pure material and a short fill. Purity tells you what fraction of what is there is the right molecule; content tells you how much of it there is. You need both, and a certificate with only purity is telling you half the story.

Last edited: Jan 22, 2025 at 4:49 PM
16 11BrianDallas92, labquiet_amy, emily_PDX and 13 others
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Sigma-Aldrich — Research-Grade Standards

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PharmD_Rodriguez
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Jan 22, 2025 at 7:51 PM#9

OP back with an update, since a thread like this is useless without one.

Went back through my own correspondence with an earlier supplier and the warning signs were all there in the replies, weeks before the quality changed. I was reading the product and not the behaviour.

Last edited: Jan 23, 2025 at 12:51 AM
15 10dan_philly, MeganSA_TX, LarryQC_SD and 12 others
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MikeNYC_runner
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Jan 24, 2025 at 3:15 PM#10
NurseAsh_DET said:
They are not contradictory, they measure different things, and this is the single most common misreading of a certificate.

Right, and one flattering data point from a group buy is not consistency. Consistency means separate batches, separately commissioned, over months.

Last edited: Jan 24, 2025 at 5:15 PM
15 13MASHdoc_SA, GenomicsKate, Dr.ObesityMed and 12 others
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